硫酸软骨素钠在ChromCore SAX-Chs色谱柱上的分离(中国药典)

Column: ChromCore SAX-Chs, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 水, pH3.5
B) 2 mol/L氯化钠溶液, pH3.5
Gradient: t (min) %A %B
0 100 0
4 100 0
45 50 50
Flow Rate: 0.8 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 232 nm
Sample: 硫酸软骨素供试品
Peaks: 1. 软骨素二糖
2. 6-硫酸化软骨素二糖
3. 4-硫酸化软骨素二糖