牛血清白蛋白在ChromCore 300 C4-T色谱柱上的分离

Column: ChromCore 300 C4-T, 5 μm
Dimension: 4.6×50 mm
Mobile Phase: A) 0.1% TFA in H20
B) 0.1% TFA in MeCN
Gradient: t (min) %A %B
0 75 25
8 40 60
9 75 25
Flow Rate: 1.0 mL/min
Temperature: 70 °C
Injection: 10 μL
Detection: UV 280 nm