耗材描述

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+.

Cat No Size Price($) A1201-200 200 units 75 A1201-500 500 units 155

The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.   Applications - First- and second-strand synthesis of cDNA. - Primer extensions and RNA sequencing - RT-PCR(a). Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase.

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