产地：MBI 

货号：B69

Quality Control

The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests. Functionally tested for the capacity to join cohesive- and blunt-end DNA fragments.

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids (1, 2).

The T4 DNA Ligase requires ATP as cofactor.