产地：MBI 

货号：EN0201

Applications

Removal of mRNA prior to synthesis of second strand cDNA (1).

RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis.

Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) (2).

Site-specific cleavage of RNA (3).

Studies of in vitro polyadenylation reaction products (4).

Description

Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids, see Fig.1. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.