产地：MBI 

货号：EN0531

Applications

Plasmid and genomic DNA preparation (3, 4).

Removal of RNA from recombinant protein preparations.

Ribonuclease protection assays. Used in conjunction with RNase T1 (3).

Mapping single-base mutations in DNA or RNA (5, 6).

Description

RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues, see Fig.1.

It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (1, 2).