产地：MBI 

货号：EP0081

Applications

Purification of covalently closed circular DNA by removal of residual genomic DNA.

Primer extension reactions on long templates (1).

DNA 3'-end labeling (1).

Strand extensions in site-directed mutagenesis (2).

Fill-in blunting of 5'-overhang DNA.

Second strand synthesis of cDNA (3).

In situ detection of DNA fragmentation associated with apoptosis (4).

Description

T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'=>3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'=>5' exonuclease activity towards single- and double-stranded DNA.