Alpha-fetoprotein(C-term)
Cat.#: 1757-1
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P02771
Mol Weight: 70kDa
Size: 100ul

Description
Alpha-fetoprotein (AFP) is a serum glycoprotein protein produced in the liver or yolk sac of fetal staged mammals. AFP synthesis is minimal after birth and a trace amount is expressed in the adult liver (1). AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways (2). AFP functions as a binding and transporting ligand and cell growth regulator. An elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. A high level of AFP is also seen in some diseases, such as hepatitis and colitis. AFP is used as a screening marker for fetal abnormalities in pregnant women, such as Down syndrome. Recently, AFP has been introduced as an anti-cancer drug-ligand carrier, transporting drugs to target tumor cells, increasing anti-tumor efficiency (3).

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:5,000
IHC: 1:100-250

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on HepG2 cell lysate of using anti-AFP (C-term) Rabbit Polyclonal (cat. # 1757-1), dilution 1:50,000
B. Immunohistochemical analysis of paraffin-embedded human fetal liver using anti-AFP Rab pAb (cat. #1757-1).
Specificity
A synthetic peptide corresponding to residues in C-terminus of human AFP was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AFP antibody, FETA antibody, HPAFP antibody, Alpha-fetoprotein antibody, Alpha-1-fetoprotein antibody, Alpha-fetoglobulin antibody

Description References
1. Wang XW, et al. World J Gastroenterol 7:345-351, 2001.
2. Chen H, et al. Crit Rev Eukaryot Gene Expr, 7:11-41, 1997.
3. Zheng AI, et al. CJC 22:108-111, 2003.