ALOX12 Antibody(C-term)
Cat.#: T3533
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P18054
Mol Weight: 76kDa
Size: 100ug

Description
Oxygenase and 14,15-leukotriene A4 synthase activity.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
IHC: 1:50~100
ELISA: 1:1,000

Species Reactivity
Human

Products Data  

 

Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human skin tissue reacted with PSMD13 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specificity
This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627~656 amino acids from the C-terminal region of human ALOX12 .

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names
ALOX12 antibody, 12-LOX antibody, 12S-LOX antibody, LOG12 antibody, Arachidonate 12-lipoxygenase, 12S-type antibody, Platelet-type lipoxygenase 12 antibody

Description References
Yoshimoto,T., et.al., Biochem. Biophys. Res. Commun. 172 (3), 1230-1235 (1990)