ATG9A antibody
Cat.#: 2975-1
Rabbit Monoclonal Antibody
Clone ID: EPR2450(2)
Swiss Prot: Q7Z3C6
Mol Weight: 105kDa
Size: 100ul

Description
Autophagy is a degradation system of cytoplasmic proteins and organelles via formation of double-membrane vesicles called autophagosomes (1). Defects in autophagy are associated with various human diseases including cancer and neurodegeneration (2). ATG9 functions in autophagy as the only integral membrane protein that is essential for double-membrane vesicle formation (2). It is probably transported to the site of autophagosome formation, the pre-autophagosomal structure (PAS) (2). One member of the ATG9 family, ATG9A (Autophagy-related gene 9A), is an autophagy-related membrane protein that is required for autophagosome formation (3, 4). Although ATG9A is ubiquitously detected, it is highly expressed in neurons of the central nervous system (4).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:50 - 100
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) HepG2, (B) 293T, (C) A375, (D) mouse brain, and (E) rat brain cell lysates using anti-ATG9A RabMAb (cat. # 2975-1).
Immunohistochemical staining of paraffin-embedded human colon tissue using anti-ATG9A RabMAb (cat. #2975-1).
Immunofluorescent staining of HepG2 cells using anti-ATG9A RabMAb (cat. #2975-1).

Specificity
A synthetic peptide corresponding to residues in human ATG9A was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATG9A antibody, APG9L1 antibody, MGD3208 antibody, mATG9 antibody, Autophagy-related protein 9A antibody, APG9-like 1 antibody, mATG9 antibody

Description References
1. Sekito T et al. Genes Cells. 14(5):525-38, 2009. 2. Reggiori F et al. Autophagy. 1(2):101-9, 2005. 3. The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. D142-D148 (2010). 4. Tamura H et al. J Histochem Cytochem. 2010.