产地：QIAGEN
货号：12145
名称：Plasmid Midi Kit

货号	产品名称	厂家	规格	单价	详细	订购
12145	QIAGEN Plasmid Midi Kit (100)	Qiagen	100T	￥9,930.00	详细查看	订购

Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart "Comparison of QIAGEN Plasmid Kit Procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation. 
Principle
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation (see figure "Ultrapure Plasmid DNA Yielded by QIAGEN Plasmid Kits"). Pre-packed QIAGEN-tips (see figure "QIAGEN Plasmid Kit Anion-Exchange Tips") operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications such as transfection (see figure "Transfection Efficiency vs Plasmid Purification Method"), cloning, manual or automated sequencing, including capillary sequencing, and in vitro transcription.
产地：QIAGEN
货号：12145
名称：Plasmid Midi Kit