Featuring ultra-efficient 4μm particles, TSKgel SuperSW3000 provides higher efficiency than 5μm TSKgel G3000SWxl columns. The 250Å pore size results in a fractionation range up to 500,000 Da for globular proteins. The narrow 4.6mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications. 

 

To fully benefit from the high efficiency of 4μm columns, and because the column diameter of TSKgel SuperSW columns is 4.6mm or less, it is important to minimize the amount of dead volume in the HPLC system. Consult the Separation Report for details (provided below).

 

Product Attributes:

 

 Base material: Silica

 Particle size (mean): 4µm

 Pore size (mean): 250Å

 Functional group: Diol

 pH stability: 2.5 - 7.5

 Calibration range: 10,000-500,000 Da(globular proteins)

 

应用实例

 

用 0.2M NaCl, 0.2M NaClO4和 0.4M NaClO4，单克隆抗体的分离

 

(Enlargement of fusion protein 1's baseline region shows improved peak shape of the dimer and trimer aggregates with 0.4M NaClO4)

 

 

Published with permission of Scott Lauder, Lexigen Pharmaceuticals