刀豆凝集素A(Con A)
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分子式 分子量 Beilstein号 EC号 234-258-2 MDL号 MFCD00071069 PubChem编号 属性 溶解性 Soluble in water(slightly hazy), and PBS (5 mg/mL, s
| 货号 | CAS号 | 操作 |
|---|---|---|
| C100881-100mg | 11028-71-0 | 询底价 |
| C100881-1g | 11028-71-0 | 询底价 |
| C100881-250mg | 11028-71-0 | 询底价 |
| C100881-25mg | 11028-71-0 | 询底价 |
耗材描述
- 分子式
- 分子量
- Beilstein号
- EC号 234-258-2
- MDL号 MFCD00071069
- PubChem编号
属性
| 溶解性 | Soluble in water(slightly hazy), and PBS (5 mg/mL, slightly hazy). |
| 存贮条件 | 储存温度-20°C |
描述
| 应用 |
它为促细胞有丝分裂素(mito-gen)，主要对T淋巴细胞有激发作用，凝集红细胞和动物精子，抑制肿瘤细胞运动，以及延长异源移植存活时间等作用。一种重要的生化和免疫研究试剂。 Appearance: White lyophilized powder Source: Jack beans Molecular weight: 104 kDa Sugar specificity: a-Man, a-Glc Activity: Con A reacts with a number of bacterial and animal cells and can distinguish between certain normal and tumour cells. It can initiate cell division (mitogenesis). 0,5-10 μg/ml is required to visibly agglutinate neuraminidase treated erythrocytes. Microorganisms: < 100 CFU/g Protein content: > 90 %, OD280nm (ε 1mg/ml = 1.14), > 95%, essentially salt free |
| 产品介绍 | 自刀豆和豌豆等籽粒中提取而得的一种植物凝集素(1ectin)。为四聚体球蛋白，分子量102000。每个亚基含237个氨基酸残基，分子量25500，结合一个Ca2+和一个Mn2+，含一个糖结合部位。能与α-甘露糖、α-葡萄糖(细胞膜糖蛋白上)专一地结合，结合位点要求是α-D-吡喃甘露糖或α-D吡喃葡萄糖六元环中的C-3/C-4和C-6未被取代羟基。能与以α-1，2糖苷键连接的甘露二糖和甘露三糖有最大的亲和力。 Concanavalin A is a plant lectin that is a T cell mitogen. It induces apoptosis in human fibroblasts. Concanavalin A (Con A) lectin is isolated from Jack beans (Canavalia ensiformis) and purified by affinity chromatography. The molecular weight of the lectin is 104 kDa. It has broad applicability and is the most widely used lectin within molecular biology research. Con A binds specifically to α-Mannose, α-Galactose structures found in sugars, glycoproteins and glycolipids (2). The lectin has been utilized in hormone receptor studies, mitogenic assays and for characterizing normal and malignant cells (cancer cells are readily aggregated by Con A while normal cells are not). Con A can also initiate cell division (mitogenesis) principally acting on T-lymphocytes (3). Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures. Polyacrylamide gel electrophoresis in SDS of Con A from Jack beans yields three major bands corresponding to molecular weights 27, 13 and 10 kDa. One minor band is visible at 18 kDa. (4). Con A is supplied as a white lyophilized powder from a buffer containing 0.5 mM MnCl2, 0.5 mM CaCl2, no preservatives are added. The identity is determined by SDS-PAGE (Figure 1) and the protein content by spectrophotometry (Figure 2). The lectin is available in vials containing 250 mg or 100 mg lyophilized powder and the product is to be used for laboratory work only. |
| 生化机理 | 刀豆素 A 无血型特异性，但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。在 pH 6.5 或更低的条件下解离为二聚体,在 pH 5.8 至 7.0 之间时，以四聚体形式存在,pH 高于 7.0 时，形成更高的聚合体。根据聚合程度不同，可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一?种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。 |
| 别名 | 伴刀豆凝集素;伴刀豆球蛋白A;刀豆球蛋白A;刀豆素A;;Con A |
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