TRIzol® Plus RNA 纯化系统
无与伦比的裂解能力及最高的产量

1.TRIzol® 试剂样本裂解
2.PureLink® RNA 小量提取试剂盒 1,000μg 的柱结合容量，有助于获得无与伦比的产量和纯度

TRIzol Plus RNA 纯化试剂盒结合了 TRIzol 试剂强大的裂解能力和 PureLink™ RNA 小提试剂盒便捷、省时的硅胶纯化柱方案，通常可在一小时内从多种样品类型中提供超纯总 RNA。

The TRIzol™ Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol™ Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink™ RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Features of this kit include:

• Combined TRIzol™-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol

Two products in one
The TRIzol™ Plus RNA Purification System couples the lysis capabilities of TRIzol™ Reagent with the 1000 µg column-;binding capacity of the PureLink™ RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol™ Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol™ Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol™ Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol™ Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol™ Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink™ RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).

规格

产品规格：离心柱

分离技术：Silica Spin Column, Organic Extraction

Purification Time：20 min.

样品类型：植物样品、酵母菌、组织、细菌、细胞

最终产品类型：Total RNA

高通量能力：不兼容高通量应用（手动）

适用于（应用）：Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nucleic Acid Labeling, Nuclease Protection Assays, Northern Blotting

反应次数：50 次制备

产品线：TRIzol™

数量：50

规格：小型

原始材料量：多达 5 x 10^8 个细胞，多达 200 mg 组织

靶标：Total RNA

系统类型：TRIzol

产量：1000 µg

内容与储存

试剂盒组成部分：
• TRIzol™ 试剂 (100 mL)
• 裂解缓冲液 (125 mL)
• 洗涤缓冲液 I (50 mL)
• 洗涤缓冲液 II (15 mL)
• 无 RNase 水 (15.5 mL)
• 带收集管的离心柱（各 50 个）
• 收集管（各 50 个）
• 回收管（各 50 个）

所有组分均储存在室温下。提供足够进行 50 次 RNA 分离的试剂。