For High-Resolution Separations of Free Amino Acids by AAA-Direct

The AminoPac^®PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct^™Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.

• Derivatization not required

• Carbohydrates and amino acids separated and detected simultaneously

• Mid-femtomole to low-picomole detection limits

• Compatible with all commonly used hydrolysis procedures

• Can be configured for on-line analysis

AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths.

The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.

Determination of amino acids and carbohydrates in LB broth supernatant.

Amino acids and sugars in red wine.

"High Glucose" Fermentation Broth (1:250 Dilution)

Separation of an Amino Acid Standard Mixture Containing 100 pmol of Each Component.

Comparison of Amino Acid Concentrations for Collagen Measured by Integrated Amperometry and Ninhydrin Methods.