赛默飞Thermo CarboPac MA1 Carbohydrate Column蛋白质分析专用柱
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CarboPac MA1 Column for Carbohydrate, Alditol, and Aldose AnalysisThe CarboPac®MA1 column is a high-capacity, strong anio
CarboPac MA1 Column for Carbohydrate, Alditol, and Aldose Analysis
The CarboPac®MA1 column is a high-capacity, strong anion-exchange column that delivers sensitive, rugged, and reliable separations of reduced sugars without the need for derivatization. The MA1 consists of a macroporous resin fully functionalized with a tertiary amine.
Recommended for the separation of reduced mono- and disaccharides
No derivatization needed
Separations using a simple hydroxide eluent
Alditols and aldoses separated from one another in a single run
Designed for reduced mono- and disaccharide alditol analyses, the CarboPac MA1 is also well suited for analysis of exoglycosidase-released neutral monosaccharides. Monosaccharides are moved away from the column void, enabling immediate evaluation of contaminating exoglycosidase activities. The MA1 can provide baseline resolution of fucose, N-acetyl-(D)-glucosamine, N-acetyl-galactosamine, mannose, glucose, and galactose, as well as neutral oligosaccharides in the same separation.
| CarboPac MA1 Column for Carbohydrate Alditol and Aldose AnalysisSpecifications | |
|---|---|
| Dimensions | CarboPac MA1 Analytical Column: 4×250 mm CarboPac MA1 Guard Column: 4×50 mm |
| Maximum operating pressure | 2000 psi (14 MPa) |
| Temperature range | 4–50 °C Recommended operating temperature: ambient |
| Typical operating conditions | Recommended flow rate: 0.2–0.5 mL/min |
| Mobile phase compatibility | pH 0–14; incompatible with solvents and anionic detergents. Hydroxide eluents only. |
| Resin composition | 7.5-µm-diameter vinylbenzyl chloride/divinylbenzene macroporous substrate fully functionalized with an alkyl quaternary ammonium group (15% cross-linked). |
| Anion-exchange capacity | Approximately 1450 µeq/column (4×250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
Gradient separation of alditols and aldoses potentially released by hydrolysis of beta eliminated oligosaccharides. Arabitol serves as an internal standard.
HPAE separation of sugars and sugar alcohols without the need for derivatization.
Isocratic separation of a representative set of alditols and aldoses ensures lot-to-lot reproducibility in the manufacturing of the CarboPac MA1 column.
| Analytical Columns | |
|---|---|
| CarboPac MA1 Analytical Column (4 x 250 mm) | 044066 |
| Guard Columns | |
|---|---|
| CarboPac MA1 Guard Column (4 x 50 mm) | 044067 |
