High-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of Proteins

The ProPac^®SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anion exchange surface provides pH control, selectivity, and fast mass transfer for high efficiency separation, as well as moderate capacity.

• Unequalled resolution

• High-efficiency peaks with excellent recovery

• Higher capacity than other columns based on nonporous particles

• Useful for characterization and quality control assessment of closely-related protein variants

• Supports separation of proteins that differ by as little as one amino acid residue

• Neutral hydrophilic coat that eliminates protein-resin hydrophobic interactions

• Superior lot-to-lot and column-to-column reproducibility

ProPac SAX-10 and WAX-10 columns can resolve protein isoforms that differ by as little as one charged residue. Differences in selectivity between the WAX-10 and SAX-10 provide flexibility in maximizing resolution of closely related species.

All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. Dionex also offers these columns as lot select packages, containing three columns from one lot or three columns from three different lots, to assist quality control scientists in method validation.

Resolution of phosphorylation variants of ovalbumin.

Effect of sialylation on transferrin chromatography.

Profiling dairy milk caseins.

The ProPac WAX shows high selectivity for proteins in this separation of carbonic anhydrase.