N109040-1g2-硝基苯基-β-D-吡喃半乳糖苷(ONPG)
面议
- 阿拉丁
- 生产商
- N109040-1g
- 现货
- 全国
规格 |
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耗材描述
- 分子式 C12H15O8N
- 分子量301.25
- Beilstein号 92207
- EC号 206-716-1
- MDL号 MFCD00063255
- PubChem编号 24897461
属性
熔点 | 195°C |
旋光率 | -68.8 ° (C=1, H2O) |
存贮条件 | -20℃充氩保存 |
密度 | 1.5990 |
描述
应用 | 用于检测β-半乳糖苷酶(β-galactosidase)活性,是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物,在410-420nm波长可以检测吸光度。 |
产品介绍 | 溶于水、甲醇,水中溶解度可达15mg/ml。有吸湿性。 |
生化机理 | β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period. |
别名 | ONPG;邻硝基苯-β-D-吡喃半乳糖苷;o-Nitrophenyl β-D-galactopyranoside ONPG |
个人防护设备 | Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter |
WGK德国 | 3 |
安全声明(欧洲) | 22,24/25 |
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