ProPacSAXandWAX蛋白质阴离子交换色谱柱,弱阴离子交换柱WAX/DEAE

概述:
The ProPac^® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anion exchange surface provides pH control, selectivity, and fast mass transfer for high efficiency separation, as well as moderate capacity.

Unequalled resolution
High-efficiency peaks with excellent recovery
Higher capacity than other columns based on nonporous particles
Useful for characterization and quality control assessment of closely-related protein variants
Supports separation of proteins that differ by as little as one amino acid residue
Neutral hydrophilic coat that eliminates protein-resin hydrophobic interactions
Superior lot-to-lot and column-to-column reproducibility

ProPac SAX-10 and WAX-10 columns can resolve protein isoforms that differ by as little as one charged residue. Differences in selectivity between the WAX-10 and SAX-10 provide flexibility in maximizing resolution of closely related species.

All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. Dionex also offers these columns as lot select packages, containing three columns from one lot or three columns from three different lots, to assist quality control scientists in method validation.

规格:

ProPac SAX-10 Strong Anion-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications
Maximum operating pressure	3000 psi (21 MPa)
Maximum operating conditions	60 °C Recommended operating temperature: <40 °C
Typical operating conditions	UV detection at 1 mL/min Recommended flow rate: 1 mL/min
Mobile phase compatibility	pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and zwitterionic detergents. Incompatible with anionic detergents.
Resin composition	10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the quaternary ammonium functional groups.
Anion-exchange capacity	< 100 µg protein on-column, depending upon the protein (4 × 250 mm analytical column)
Column construction	PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.

ProPac WAX-10 Weak Anion-Exchange Column for the High-Resolution Analysis of Protein Variants Specifications
Maximum operating pressure	3000 psi (21 MPa)
Maximum operating temperature	60 °C Recommended operating temperature: <40 °C
Typical operating conditions	UV detection at 1 mL/min Recommended flow rate: 1 mL/min
Mobile phase compatibility	pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and switterionic detergents. Incompatible with anionic detergents.
Resin composition	10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the tertiary amine functional groups.
Anion-exchange capacity	< 100 µg protein on-column, depending upon the protein (4 × 250 mm analytical column)
Column construction	PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.

订货信息:

SAX Analytical Columns
ProPac SAX-10 Analytical Column (2 x 250 mm)	063448
ProPac SAX-10 Analytical Column (4 x 250 mm)	054997
ProPac SAX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin)	SP5729
ProPac SAX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin)	SP5730
ProPac SAX-10 Semi-Preparative Column (9 x 250 mm)	063703
ProPac SAX-10 Semi-Preparative Column (22 x 250 mm)	SP5594

WAX Analytical Columns
ProPac WAX-10 Analytical Column (2 x 250 mm)	063464
ProPac WAX-10 Analytical Column (4 x 250 mm)	054999
ProPac WAX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin)	SP5731
ProPac WAX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin)	SP5732
ProPac WAX-10 Semi-Preparative Column (9 x 250 mm)	063707
ProPac WAX-10 Semi-Preparative Column (22 x 250 mm)	SP5598

WAX Guard Columns

ProPac WAX-10G Guard Column (2 x 50 mm)	063470
ProPac WAX-10G Guard Column (4 x 50 mm)	055150

SAX Guard Columns
ProPac SAX-10G Guard Column (2 x 50 mm)	063454
ProPac SAX-10G Guard Column (4 x 50 mm)	054998

应用谱图：

Resolution of phosphorylation variants of ovalbumin.

Effect of sialylation on transferrin chromatography.

Profiling dairy milk caseins.

The ProPac WAX shows high selectivity for proteins in this separation of carbonic anhydrase.

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