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订购MBIER0991;AatII现货原装

订购MBIER0991;AatII现货原装
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产地:MBI 

货号:ER0991


Conditions for 100% Activity

1X Buffer Tango™:

33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1mg/ ml BSA.

Incubate at 37°C.

Storage Buffer

AatII is supplied in:

10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Digestion of Agarose-embedded DNA

Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Ligation and Recleavage

After 50-fold overdigestion with AatII, more than 95% of the DNA fragments can be ligated and recut.