产地：QIAGEN
货号：12163
名称：Plasmid Maxi Kit
Principle
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation (see figure "Ultrapure Plasmid DNA Yielded by QIAGEN Plasmid Kits"). Pre-packed QIAGEN-tips (see figure "QIAGEN Plasmid Kit Anion-Exchange Tips") operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications such as transfection (see figure "Transfection Efficiency vs Plasmid Purification Method"), cloning, manual or automated sequencing, including capillary sequencing, and in vitro transcription.
Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart "Comparison of QIAGEN Plasmid Kit Procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation. 

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途