QIAGENPlasmidMegaKit;QIAGEN12181正品热销,质粒提取纯化

Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart "Comparison of QIAGEN Plasmid Kit Procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications such as transfection (see figure "Transfection Efficiency vs Plasmid Purification Method"), cloning, manual or automated sequencing, including capillary sequencing, and in vitro transcription.
Principle
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation (see figure "Ultrapure Plasmid DNA Yielded by QIAGEN Plasmid Kits"). Pre-packed QIAGEN-tips (see figure "QIAGEN Plasmid Kit Anion-Exchange Tips") operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
QIAGEN Plasmid Mega Kit
纯化最高产量可达 2.5 mg的转染级别纯度的质粒或科斯质粒DNA
纯度相当于 2倍氯化铯梯度离心
高重现性的产量和转染级别纯度
经济实惠
使用LyseBlue，获得最佳的裂解和最大的DNA产量
传统的QIAGEN Plasmid Giga Kits应用重力流阴离子交换柱QIAGEN-tip 2500 高效纯化质粒DNA，也单独提供QIAGEN-tips 2500。
QIAGEN Plasmid Mega Kit 特性
预期产量柱子容量培养体积
Up to 2.5 mg 2.5 mg 500 ml - 2.5 liters
实际产量取决于质粒拷贝数、插入片段大小、宿主菌、培养基和培养体积。
培养体积取决于质粒拷贝数、插入片段大小、宿主菌和培养基
QIAGEN 独特的阴离子交换柱专用于核酸纯化，其卓越的抽提性能可确保DNA纯度相当于甚至高于两次CsCl梯度离心的产物（见图"QIAGEN Plasmid kits制备超纯的质粒DNA）。预装的QIAGEN柱子以重力流方式纯化，不会流干，将质粒制备所需的手工操作时间降至最少。整个质粒纯化系统避免了苯酚、氯仿、ethidium bromide和CsCl等有毒物质，将对用户和环境的危害降至最低。
应用QIAGEN Plasmid Kits纯化过程中，通过离心取出细菌裂解液。然后将澄清的裂解液装入阴离子交换柱，在适当的低盐浓度和pH值条件下，阴离子交换柱会选择性地结合质粒DNA。RNA、蛋白质、代谢物和其他小分子杂质通过中盐浓度洗涤去除，应用高盐浓度缓冲夜洗脱超纯的质粒DNA,通过异丙醇沉淀浓缩并脱盐DNA，离心法收集DNA。
产地：QIAGEN
货号：12181
名称：QIAGEN Plasmid Mega Kit
订购电话:021-51870956
在线QQ:470003480
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