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聚合酶

Herculase II Fusion DNA 聚合酶

品牌 厂商性质 产地 货期
安捷伦 生产商 美国 现货

性能特点

Herculase II Fusion DNA 聚合酶实现优异产率,延伸时间短达 15 秒/kb,即使基因组 DNA 靶标长达 23 kb 亦是如此。Herculase II 克服 PCR 挑

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产品参数
货号 规格 操作
600675 Herculase II Fusion DNA Polymerase 询底价
600677 Herculase II Fusion DNA Polymerase 询底价
600679 Herculase II Fusion DNA Polymerase 询底价
产品介绍

Herculase II Fusion DNA 聚合酶实现优异产率,延伸时间短达 15 秒/kb,即使基因组 DNA 靶标长达 23 kb 亦是如此。Herculase II 克服 PCR 挑战,成功扩增所有复杂度的靶标。轻松扩增低丰度靶标、高 GC 含量靶标和其他棘手靶标,产率优异。
  • 对于常规 PCR 应用和难扩增/富含 GC 的靶标,产率优异
  • 循环时间快,延伸速率为 15 秒/kb
  • 准确扩增长达 23 kb 的基因组 DNA 靶标
  • 保持 1 个错误/777000 bp 的 Pfu 保真度
  • 对于扩增少量起始材料,灵敏度高
  • 该产品可根据您的需求进行定制。
    技术
    工作原理

    Successful amplification of GC-rich and complex targets

    A specially engineered enzyme and optimized buffer system enable the Herculase II enzyme to amplify difficult targets with high yields using fast cycling conditions. Targets that contain as much as 84% GC content are easily amplified whereas other competitors’ enzymes failed or produced low yield. To enhance amplification of problematic or GC-rich templates, DMSO (provided in a separate tube) can be added (3–10% DMSO; titrated in 1% increments to find optimal performance with your targets).


    High sensitivity with low abundance targets

    Herculase II amplifies DNA fragments with high sensitivity: a 3.9 kb fragment of the human a1-antitrypsin gene is amplified from as little as 1 ng input human genomic DNA.

    It is an ideal polymerase for NGS. The high yields allow for fewer cycles of amplification, reducing PCR bias. In addition, during library preparation, Herculase II delivers the best size distribution of any polymerase with most amplicons in the ideal sequencing size range. With SureSelect, this leads to the need for fewer sequencing reactions and better sets of sequencing data.
     


    Exclusive ArchaeMaxx Factor

    The result of fusing a DNA polymerase with a high-affinity double-stranded DNA binding domain, Herculase II offers increased processivity with better anchoring, preventing early dissociation from a DNA template.

    ArchaeMaxx factor was isolated from Pyrococcus furiosus and added to the enzyme. ArchaeMaxx improves the Pfu DNA polymerase yield by overcoming dUTP poisoning caused by dUTP accumulation during PCR. This poisoning inhibits Pfu, limiting its efficiency. ArchaeMaxx functions as a dUTPase, converting toxic dUTP to dUMP and inorganic pyrophosphate."

     


    资料库
    产品样本

    Surely better - PCR Solutions - SureCycler 8800_5990-7749EN

    Surely better PCR: Functionality for your lab

    目录

    Herculase II Fusion DNA 聚合酶信息由安捷伦科技(中国)有限公司为您提供,如您想了解更多关于Herculase II Fusion DNA 聚合酶报价、型号、参数等信息,欢迎来电或留言咨询。

    注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途