寡核苷酸在DNACore NP-Q色谱柱上的分离

寡核苷酸
Column: DNACore NP-Q, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 90/10 v/v 0.05 M NH3·H2O/ACN
B) 1.25 M NaCl in mobile phase A
Gradient: t (min) %A %B
0 65 35
20 35 65
20.1 0 100
25 0 100
25.1 65 35
35 65 35
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Samples: 样品1 (58 pmol/μL, 用0.1%氨水溶解)
样品2 (18.7 pmol/μL, 用0.1%氨水溶解)