脂质在ChromCore C30色谱柱上的分离

脂质
Column: ChromCore C30, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) MeCN
B) 100 mM NH4OAc, pH5
C) IPA
Gradient: t (min) %A %B %C
-10 85 5 10
0 85 5 10
10 65 5 30
60 20 5 75
70 5 5 90
80 5 5 90
Flow Rate: 1.0 mL/min
Temperature : 30 °C
Injection: 5 μL
Detection: CAD
Sample: Cooking oils (5 mg/mL)