四种生育酚
Column: ChromCore C30-VE专用柱, 3 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 水
B) 甲醇
Gradient: t(min) %A %B
0 17 83
15 17 83
15.5 7 93
22 7 93
32 5 95
45 5 95
45.5 17 83
60 17 83
Flow Rate: 1.0 mL/min
Temperature: 25 °C
Injection: 10 μL
Detection: UV 294 nm
Samples: 混标
Peaks: 1. Retinol
2. δ-Tocopherol
3. γ-Tocopherol
4. β-Tocopherol
5. α-Tocopherol