蛋白
Column: ChromCore 300 C4, 3 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 0.1% 三氟乙酸水
B) 0.1% 三氟乙酸乙腈
Gradient: t(min) %A %B
0 68 32
30 32 68
31 68 32
40 68 32
Flow Rate: 1.0 mL/min
Temperature: 35 °C
Injection: 10 μL
Detection: UV 214 nm
Sample: 蛋白 (1 mg/mL)