双特异性抗体
Column: BioCore WCX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM MES, pH6.5
B) 500 mM NaCl in 20 mM MES, pH6.5
Gradient: t (min) %A %B
0 83 17
40 68 32
40.01 0 100
45 0 100
45.01 83 17
65 83 17
Flow Rate: 0.5 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: 双特异性抗体 (~9 mg/mL )