IgG抗体
Column: BioCore SAX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM Tris, pH8.5
B) 500 mM NaCl in 20 mM Tris, pH8.5
Gradient: t(min) %A %B
0 78 22
40 72 28
40.1 0 100
45 0 100
45.1 78 22
65 78 22
Flow Rate: 0.5 mL/min
Temperature: 30 °C
Injection: 5 μL
Detection: UV 214 nm
Samples: 抗体13标准品 (2 mg/mL)
抗体13供试品 (4.35 mg/mL)