质粒在DNACore NP-Q色谱柱上的分离

质粒
Column: DNACore NP-Q, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM Tris, pH7.5
B) 1 M NaCl in 20 mM Tris, pH7.5
Gradient: t(min) %A %B
0 100 0
2 35 65
27 15 85
28 100 0
35 100 0
Flow Rate: 0.5 mL/min
Temperature: 30 °C
Injection: 20 μL
Detection: UV 260 nm
Sample: 质粒 (100 ng/μL)