ADC与Pro蛋白
Column: BioCore HIC-Butyl, 5 μm
Dimension: 4.6×100 mm
Mobile Phase: A) 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0
B) 100 mM phosphate buffer, pH7.0
C) Isopropanol
Gradient: t(min) %A %B %C
0 50 50 0
1 50 50 0
15 0 75 25
20 0 75 0
20.1 50 50 0
35 50 50 0
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 20 μL
Detection: UV 220 nm
Samples: ADC (0.46 mg/mL)
Pro蛋白 (1.8 mg/mL)