吐温80在ChromCore SAA色谱柱上的分离

吐温80
Column: ChromCore SAA, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 0.1%乙酸异丙醇溶液
B) 0.1%乙酸水溶液
Gradient: t(min) %A %B
0 10 90
0.1 10 90
0.2 34 66
2.6 34 66
2.7 100 0
6.7 100 0
6.8 10 90
15 10 90
Flow Rate: 1.2 mL/min
Temperature: 25 °C
Injection: 5 μL
Detection: ELSD: Evap:65 °C，Neb:55 °C, Gas:1.6SLM
Sample: 吐温80
Peak: 1. 吐温80