Column: ChromCore 120 C18, 3 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 85/15 v/v 醋酸铵-高氯酸溶液, pH2.2/乙腈
B) 乙腈
Gradient: t (min) %A %B
-10 100 0
0 100 0
18 100 0
25 70 30
39 70 30
40 100 0
Flow Rate: 1.0 mL/min
Temperature: 40 °C
Injection: 10 μL
Detection: UV 254 nm
Sample: 左氧氟沙星水溶液