维生素D 代谢产物在 ChromCore PFP/Phenyl色谱柱上的分离

Column: ChromCore PFP, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: 65/35 v/v 甲醇/水, 其中含有0.05%甲酸 (v%)
和0.07%甲酸铵 (wt%)
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 5 μL
Detection: UV 254 nm
Peaks: 1. 25-OH Vitamin D3, 10 μg/mL
2. 3-epi-25-OH Vitamin D3, 10 μg/mL
3. 25-OH Vitamin D2, 5 μg/mL
4. 3-epi-25-OH Vitamin D2, 10 μg/mL