Column: ChromCore C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 乙腈
B) 0.05%磷酸水溶液
Gradient: t (min) %A %B
0 19 81
8 19 81
35 50 50
36 100 0
40 19 81
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 237 nm
Sample: 甘草
Peaks: 1. 甘草苷
2. 甘草酸铵