肾上腺素在ChromCore Phenyl色谱柱上的分离

Column: ChromCore Phenyl, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 水 (含0.1%磷酸和0.1%庚烷磺酸钠)
B) 70%甲醇水 (含0.1%磷酸和0.1%庚烷磺酸钠)
Gradient: t (min) %A %B
0 100 0
8 100 0
50 65 35
60 30 70
65 30 70
68 100 0
Flow Rate: 1.0 mL/min
Temperature: 25 °C
Injection: 10 μL
Detection: UV 210 nm
Sample: 肾上腺素
Peaks: 1~2. 杂质
3. 肾上腺素
4~6. 杂质