Column: ChromCore 120 C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 50/50 v/v 四氢呋喃-乙腈 (5/95)/ 0.01 M乙酸铵溶液, pH3.2
B) 20/80 v/v 四氢呋喃-乙腈 (5/95)/ 0.01 M乙酸铵溶液, pH3.2
Gradient: t (min) %A %B
0 0 100
10 15 85
15 15 85
35 85 15
36 0 100
50 0 100
Flow Rate: 1.0 mL/min
Temperature: 25 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: 那氟沙星
Peaks: 1~9. 杂质
10. 那氟沙星
11~13. 杂质