Column: ChromCore HILIC-Amide, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 乙腈
B) 0.01 mol/L磷酸二氢钠溶液
Gradient: t (min) %A %B
0 10 90
15 90 10
20 90 10
20.1 10 90
40 10 90
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 20 μL
Detection: UV 210 nm
Sample: 甲磺酰乙酸 (0.5 mg/mL 50%乙腈水溶解)
Peak: 1. 甲磺酰乙酸