Column: ChromCore AR C18, 3 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 水
B) 甲醇
Gradient: t (min) %A %B
0 90 10
5 90 10
15 63 37
30 63 37
31 0 100
41 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 243 nm
Sample: 川牛膝
Peak: 1. 杯苋甾酮