Column: ChromCore Polar C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 0.2%磷酸水
B) 乙腈
Gradient: t (min) %A %B
0 42 58
32 42 58
35 35 65
55 15 85
60 5 95
65 42 58
Flow Rate: 1.0 mL/min
Temperature: 25 °C
Injection: 10 μL
Detection: UV 208 nm
Samples: 对照溶液 (β-桉叶醇0.03 mg/mL、苍术素0.04 mg/mL、
苍术酮0.05 mg/mL )
供试品溶液 (30 mg/mL, 甲醇超声处理)
Peaks: 1. β-桉叶醇
2. 苍术素
3. 苍术酮