Column: ChromCore Polar C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 0.1%磷酸二氢钾溶液, pH2.5
B) 乙腈
Gradient: t (min) %A %B
0 65 35
10 65 35
40 50 50
52 23 77
53 65 35
62 65 35
Flow Rate: 1.0 mL/min
Temperature: 35 °C
Injection: 50 μL
Detection: UV 251 nm
Samples: 灵敏度溶液 (0.25 μg/mL)
对照溶液 (5 μg/mL)
供试品溶液 (0.5 mg/mL)
Peak: 1. 炎琥宁