Column: ChromCore AR C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 甲醇
B) 0.3%磷酸水溶液
Gradient: t (min) %A %B
0 35 65
10 35 65
20 40 60
35 50 50
Flow Rate: 0.7 mL/min
Temperature: 35 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: 王不留行
Peak: 1. 王不留行黄酮苷