5-羟甲基糠醛在ChromCore 120 C18上的分离

Column: ChromCore 120 C18, 3 μm
Dimension: 3.0×150 mm
Mobile Phase: A) 甲醇
B) 0.05%磷酸水溶液
Gradient: t (min) %A %B
0 5 95
1.70 5 95
2.27 8 92
7.37 8 92
8.51 50 50
11.34 0 100
13.04 0 100
15.31 50 50
17.01 5 95
25.00 5 95
Flow Rate: 0.45 mL/min
Temperature: 23 °C
Injection: 2.5 μL
Detection: UV 284 nm
Sample: 5-羟甲基糠醛
Peak: 1. 5-羟甲基糠醛