Column: ChromCore AR C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 乙腈
B) 0.1/0.1/100 v/v/v 三乙胺/磷酸/水
Gradient: t (min) %A %B
0 14 86
10 17 83
24 32 68
45 70 30
46 14 86
Flow Rate: 0.8 mL/min
Temperature: 20 °C
Injection: 5 μL
Detection: UV 230 nm
Sample: 白芍总苷
Peaks: 1. 芍药内酯苷
2. 芍药苷
3. 1, 2, 3, 4, 6-五没食子酰基葡萄糖