Column: ChromCore AR C18, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 乙腈
B) 水
Gradient: t (min) %A %B
0 20 80
15 25 75
30 28 72
60 40 60
65 85 15
Flow Rate: 1.0 mL/min
Temperature: 35 °C
Injection: 5 μL
Detection: UV 205 nm
Sample: 穿心莲
Peaks: 1. 穿心莲内酯
2. 新穿心莲内酯
3. 14-去氧穿心莲内酯
4. 脱水穿心莲内酯