Column: ChromCore NH2-Acarbose, 5 μm
Dimension: 4.6×10 mm (保护柱)+4.6×250 mm
Mobile Phase: 75/25 v/v Acetontrile/600 mg KH2PO4 and 279 mg
Na2HPO4 in 1000 mL Water
Flow Rate: 2.0 mL/min
Temperature: 35 °C
Injection: 10 μL
Detection: UV 210 nm
Sample: 20 mg/mL Acarbose
Peaks： 1. Acarbose Impurity IV
2. Acarbose Impurity II
3. Acarbose Impurity I
4. Acarbose
5. Acarbose Impurity III