Column: BioCore WCX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM phosphate buffer, pH6.5
B) 300 mM NaCl in 20 mM phosphate buffer, pH6.5
Gradient: t (min) %A %B
0 100 0
40 65 35
40.1 0 100
45 0 100
45.1 100 0
60 100 0
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 20 μL
Detection: UV 280 nm
Sample: 双抗