Column: BioCore Protein A, 15 μm
Dimension: 2.1×50 mm
Mobile Phase: A) 150 mM NaCl in 50 mM phosphate buffer, pH7.0
B) 150 mM NaCl in 50 mM phosphate buffer, pH2.5
Gradient: t (min) %A %B
0 100 0
0.83 100 0
0.84 0 100
2.50 0 100
2.51 100 0
4.00 100 0
Flow Rate: 2.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: 细胞澄清液