Column: BioCore WCX, 10 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 20 mM MES, pH6.5
B) 300 mM NaCl in 20 mM MES, pH6.5
Gradient: t (min) %A %B
0 80 20
1 80 20
20 20 80
20.1 0 100
25 0 100
25.1 80 20
40 80 20
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 5 μL
Detection: UV 280 nm
Sample: 重组白介素11