蛇毒蛋白在BioCore SCX色谱柱上的分离

Column: BioCore SCX, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM Tris-HCl, pH6.0
B) 300 mM NaCl in 20 mM Tris-HCl, pH6.0
Gradient: t (min) %A %B
-15 100 0
0 100 0
30 0 100
35 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: 蛇毒蛋白 (5.0 mg/mL in Mobile Phase A)